CD4 MicroBeads, human
author:admin source:admin published:2013-04-25 10:24:54
Product Name: CD4
MicroBeads, human
Background
information:
CD4 is an
accessory molecule in the recognition of MHC class II/peptide complexes by the
TCR heterodimers on CD4+ T helper cells. CD4 is expressed on T helper cells and
at a lower level on monocytes and dendritic cells. The CD4 molecule is the
receptor for the human immunodeficiency virus. The CD4 antibody recognizes most
thymocytes and about 65% of all peripheral blood T cells.
Downstream
applications:
Isolation or
depletion of CD4+ T helper cells is performed in many different fields of
research, such as infectious diseases, autoimmune diseases, allergy, asthma,
and tumor immunology. T helper cells isolated by MACS® Technology remain viable
and functional. Therefore, they can be used for further functional studies,
such as proliferation assays and cytotoxicity assays, as well as for the
generation of T cell lines. CD4+ T cells purified with CD4 MicroBeads have also
been cultured and analyzed for in vitro cytokine production.4 Furthermore, they
have been used in co‑culture experiments, e.g., for allogeneic
stimulation by dendritic cells1 or for B cell activation.
T helper cells
isolated by MACS® Tech­nology were also used for studies on viral
infections, e.g., for in vitro infections of cells with HIV, to determine the
infectivity of HIV-infected follicular dendritic cells, immune reconstitution
after anti-viral therapy, and for monitoring of immune abnormalities in
children of HIV-infected mothers8.