Product Name: CD4 (L3T4) MicroBeads, mouse

Background information

The CD4 antigen is expressed on most thymocytes and mature T helper cells, and at lower levels on a subpopulation of dendritic cells. CD4 acts as an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4⁺T helper cells. The CD4 antigen is expressed on approximately 90% of thymocytes, 25% of splenocytes, and 55% of lymph node cells.

Downstream applications

CD4⁺ T cells isolated with CD4 MicroBeads have been used for functional and phenotypical analysis of T cells in vitro andin vivo. The experiments comprised studies on T helper cell differentiation towards T_H1 or T_H2 cells, or studies on the regulatory role of cytokines in autoimmune disease and infection.³ Purified T helper cells were also used in a large panel of adoptive transfer experiments, e.g. for transfer into immunodeficient, knock-out, parasite infected, and tumor-bearing mice or to passively transfer a disease with auto-antigen-stimulated CD4⁺ T cells in an EAE mouse model. Isolated CD4⁺ T cells have also been used to study the role of costimulatory molecules, such as OX40-OX40 Ligand interactions. Furthermore, CD4⁺T cells were isolated from CNS suspensions of MHV infected mice for evaluation of their chemokine expression pattern.