Product Name: Treg Expansion Kit, mouse

The Treg Expansion Kit was developed for the efficient expansion of murine Treg cells.

Background information

This kit sustains FoxP3 expression in expanded Treg cells that were previously isolated with the CD4⁺CD25⁺ Regulatory T Cell Isolation Kit, mouse. The Treg Expansion Kit is based on MACSiBead™ Particles pre‑loaded with CD3 and CD28 antibodies. Optimal expansion of Treg cells is accomplished by using pre-loaded MACSiBead Particles and Treg cells at a bead-to-cell ratio of 3:1 and recombinant interleukin 2 (rIL-2) at a concentration of 2,000 U/mL.

Downstream applications

Expanded Treg cells can be used for any downstream application, such as adoptive transfer experiments, cytokine analysis, gene expression studies, and suppression assays.