Product Name: CD133 MicroBead Kit, human

 

The CD133 MicroBead Kit allows the single-step isolation or depletion of CD133⁺ cells.

• Hematopoietic stem cells can be isolated from peripheral blood, cord blood, bone marrow, or leukapheresis product.
• Neural progenitor cells can be isolated from single-cell suspensions derived from primary neural tissues or cell lines.
• ES and iPS cell–derived neural, endothelial, or hematopoietic progenitors can be isolated from differentiated ES or iPS cell cultures¹.
• Cancer stem cells can be isolated from single-cell suspensions derived from primary tumor tissue or cell lines.

 

Background information: 

CD133, formerly known as AC133, recognizes epitope 1 of the CD133 antigen. It is a marker that is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34^– cells as well as on a subset of CD34^brightstem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood. CD133 has also been found to be expressed on circulating endothelial progenitor cells, fetal neural stem cells, other tissue-specific stem cells, such as renal, prostate, and corneal stem cells, cancer stem cells from tumor tissues, as well as ES and iPS cell-derived cells.

There is a growing interest in CD133-expressing stem cells from normal blood or bone marrow in regenerative medicine, for example, in the field of cardiovascular, liver, or peripheral artery diseases.

The CD133 MicroBeads included in the kit recognize epitope CD133/1. For quality control staining of isolated CD133⁺ cells, the use of CD133/2 (293C3)-PE or -APC is recommended.