Product Name: CD4⁺CD25⁺CD127^dim/-Regulatory T Cell Isolation Kit II, human

The CD4⁺CD25⁺CD127^dim/– Regulatory T Cell Isolation Kit II was developed for the isolation of CD4⁺CD25⁺CD127^dim/– Treg cells from human PBMCs. The isolation is performed in a two-step procedure.

Background information

CD127, the α-chain of the IL-7 receptor, is expressed on the majority of mature T cells and plays an important role in their proliferation and differentiation. However, on regulatory T cells CD127 is absent and its expression inversely correlates with FoxP3 expression. Thus, CD127 can be used as an additional marker to discriminate between human regulatory and activated T cells.

Detailed separation procedure

First, the isolation of CD4⁺CD25⁺CD127^dim/– regulatory T cells was performed with a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for the depletion of non-CD4⁺ and CD127^high cells. In the second step, the flow-through fraction of pre-enriched CD4⁺CD127^dim/– T cells is labeled with CD25 MicroBeads for subsequent positive selection of CD4⁺CD25⁺CD127^dim/– regulatory T cells.

Downstream applications

Isolation of CD4⁺CD25⁺CD127^dim/– regulatory T cells from human PBMCs for further phenotypical or functional characterization.